Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates.
Identifieur interne : 000310 ( Main/Exploration ); précédent : 000309; suivant : 000311Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates.
Auteurs : Muhammad Ishtiaq Ali [Pakistan] ; Safia Ahmed ; Geoff Robson ; Imran Javed ; Naeem Ali ; Naima Atiq ; Abdul HameedSource :
- Journal of basic microbiology [ 1521-4028 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Champignons.
- métabolisme : Champignons.
- Dépollution biologique de l'environnement, Microbiologie du sol, Poly(chlorure de vinyle).
English descriptors
- KwdEn :
- MESH :
- chemical : Polyvinyl Chloride.
- isolation & purification : Fungi.
- metabolism : Fungi.
- Biodegradation, Environmental, Soil Microbiology.
Abstract
The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57 mg ml(-1) followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292 Da(-1)) in Molecular weight of the PVC film than control (200,000 Da(-1)) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics.
DOI: 10.1002/jobm.201200496
PubMed: 23686796
Affiliations:
Links toward previous steps (curation, corpus...)
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uniqKey="Javed I" first="Imran" last="Javed">Imran Javed</name></author><author><name sortKey="Ali, Naeem" sort="Ali, Naeem" uniqKey="Ali N" first="Naeem" last="Ali">Naeem Ali</name></author><author><name sortKey="Atiq, Naima" sort="Atiq, Naima" uniqKey="Atiq N" first="Naima" last="Atiq">Naima Atiq</name></author><author><name sortKey="Hameed, Abdul" sort="Hameed, Abdul" uniqKey="Hameed A" first="Abdul" last="Hameed">Abdul Hameed</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2014">2014</date><idno type="RBID">pubmed:23686796</idno><idno type="pmid">23686796</idno><idno type="doi">10.1002/jobm.201200496</idno><idno type="wicri:Area/Main/Corpus">000365</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000365</idno><idno type="wicri:Area/Main/Curation">000365</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000365</idno><idno 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Robson</name></author><author><name sortKey="Javed, Imran" sort="Javed, Imran" uniqKey="Javed I" first="Imran" last="Javed">Imran Javed</name></author><author><name sortKey="Ali, Naeem" sort="Ali, Naeem" uniqKey="Ali N" first="Naeem" last="Ali">Naeem Ali</name></author><author><name sortKey="Atiq, Naima" sort="Atiq, Naima" uniqKey="Atiq N" first="Naima" last="Atiq">Naima Atiq</name></author><author><name sortKey="Hameed, Abdul" sort="Hameed, Abdul" uniqKey="Hameed A" first="Abdul" last="Hameed">Abdul Hameed</name></author></analytic><series><title level="j">Journal of basic microbiology</title><idno type="eISSN">1521-4028</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biodegradation, Environmental (MeSH)</term><term>Fungi (isolation & purification)</term><term>Fungi (metabolism)</term><term>Polyvinyl Chloride (MeSH)</term><term>Soil Microbiology (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Champignons (isolement et purification)</term><term>Champignons (métabolisme)</term><term>Dépollution biologique de l'environnement (MeSH)</term><term>Microbiologie du sol (MeSH)</term><term>Poly(chlorure de vinyle) (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Polyvinyl Chloride</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Fungi</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Champignons</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biodegradation, Environmental</term><term>Soil Microbiology</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Dépollution biologique de l'environnement</term><term>Microbiologie du sol</term><term>Poly(chlorure de vinyle)</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57 mg ml(-1) followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292 Da(-1)) in Molecular weight of the PVC film than control (200,000 Da(-1)) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23686796</PMID><DateCompleted><Year>2014</Year><Month>08</Month><Day>02</Day></DateCompleted><DateRevised><Year>2014</Year><Month>01</Month><Day>02</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1521-4028</ISSN><JournalIssue CitedMedium="Internet"><Volume>54</Volume><Issue>1</Issue><PubDate><Year>2014</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Journal of basic microbiology</Title><ISOAbbreviation>J Basic Microbiol</ISOAbbreviation></Journal><ArticleTitle>Isolation and molecular characterization of polyvinyl chloride (PVC) plastic degrading fungal isolates.</ArticleTitle><Pagination><MedlinePgn>18-27</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jobm.201200496</ELocationID><Abstract><AbstractText>The recalcitrant nature of polyvinyl chloride creates serious environmental concerns during manufacturing and waste disposal. The present study was aimed to isolate and screen different soil fungi having potential to biodegrade PVC films. After 10 months of soil burial experiment, it was observed that a number of fungal strains were flourishing on PVC films. On morphological as well as on 18rRNA gene sequence and phylogenetic basis they were identified as Phanerochaete chrysosporium PV1, Lentinus tigrinus PV2, Aspergillus niger PV3, and Aspergillus sydowii PV4. The biodegradation ability of these fungal isolates was further checked in shake flask experiments by taking thin films of PVC (C source) in mineral salt medium. A significant change in color and surface deterioration of PVC films was confirmed through visual observation and Scanning electron microscopy. During shake flask experiments, P. chrysosporium PV1 produced maximum biomass of about 2.57 mg ml(-1) followed by A. niger PV3. P. chrysosporium PV1 showed significant reduction (178,292 Da(-1)) in Molecular weight of the PVC film than control (200,000 Da(-1)) by gel permeation chromatography. Furthermore more Fourier transform infrared spectroscopy and nuclear magnetic resonance also revealed structural changes in the PVC. It was concluded that isolated fungal strains have significant potential for biodegradation of PVC plastics.</AbstractText><CopyrightInformation>© 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ali</LastName><ForeName>Muhammad Ishtiaq</ForeName><Initials>MI</Initials><AffiliationInfo><Affiliation>Department of Microbiology, Quaid-i-Azam University, Islamabad, Pakistan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ahmed</LastName><ForeName>Safia</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Robson</LastName><ForeName>Geoff</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Javed</LastName><ForeName>Imran</ForeName><Initials>I</Initials></Author><Author ValidYN="Y"><LastName>Ali</LastName><ForeName>Naeem</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Atiq</LastName><ForeName>Naima</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Hameed</LastName><ForeName>Abdul</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>20</Day></ArticleDate></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Basic Microbiol</MedlineTA><NlmUniqueID>8503885</NlmUniqueID><ISSNLinking>0233-111X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>9002-86-2</RegistryNumber><NameOfSubstance UI="D011143">Polyvinyl Chloride</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001673" MajorTopicYN="Y">Biodegradation, Environmental</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005658" MajorTopicYN="N">Fungi</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011143" MajorTopicYN="Y">Polyvinyl Chloride</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012988" MajorTopicYN="N">Soil Microbiology</DescriptorName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Biodegradation</Keyword><Keyword MajorTopicYN="N">Fungi</Keyword><Keyword MajorTopicYN="N">GPC</Keyword><Keyword MajorTopicYN="N">Polyvinyl chloride</Keyword><Keyword MajorTopicYN="N">SEM</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>08</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2012</Year><Month>09</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>5</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23686796</ArticleId><ArticleId IdType="doi">10.1002/jobm.201200496</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pakistan</li></country></list><tree><noCountry><name sortKey="Ahmed, Safia" sort="Ahmed, Safia" uniqKey="Ahmed S" first="Safia" last="Ahmed">Safia Ahmed</name><name sortKey="Ali, Naeem" sort="Ali, Naeem" uniqKey="Ali N" first="Naeem" last="Ali">Naeem Ali</name><name sortKey="Atiq, Naima" sort="Atiq, Naima" uniqKey="Atiq N" first="Naima" last="Atiq">Naima Atiq</name><name sortKey="Hameed, Abdul" sort="Hameed, Abdul" uniqKey="Hameed A" first="Abdul" last="Hameed">Abdul Hameed</name><name sortKey="Javed, Imran" sort="Javed, Imran" uniqKey="Javed I" first="Imran" last="Javed">Imran Javed</name><name sortKey="Robson, Geoff" sort="Robson, Geoff" uniqKey="Robson G" first="Geoff" last="Robson">Geoff Robson</name></noCountry><country name="Pakistan"><noRegion><name sortKey="Ali, Muhammad Ishtiaq" sort="Ali, Muhammad Ishtiaq" uniqKey="Ali M" first="Muhammad Ishtiaq" last="Ali">Muhammad Ishtiaq Ali</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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